A Comprehensive Mutant Set for the Cyanobacterium Synechococcus Elongatus PCC 7942

Case Number: 2607TAMU08


Blue-Green Algae Gene Mining and Evaluation Clone Sets

Description of Invention:
     This is a one-of-a-kind collection of approximately 3,000 clones of genetically-engineered bacterial cells of the blue green alga (cyanobacterium) Synechococcus elongatus PCC 7942 and 17,000-30,000 clones of PCC 7942 DNA archived in the bacterium Escherichia coli. Each cyanobacterial clone carries an insertion mutation in a single gene. Collectively, the blue green algal clone set represents mutations of each gene in the entire S. elongatus genome. This set makes it possible to explore and mine the entire blue green algal genome one gene at a time.
     The E. coli clones set carry segments of the S. elongatus genome in plasmid or cosmid cloning vectors, and each of these clones carries a transposon insertion. The plasmids/cosmids from the E. coli clones are used to generate the mutant cyanobacterial strains by genetic transformation. The E. coli clone collection contains several fold more mutations than are required to inactivate each gene in the genome. A streamlined clone set of one mutation per gene, a unigene set, is used to create the entire set of S. elongatus clones.

Inventor:
Dr Susan Golden
Distinguised Professor
Texas A&M Department of Biology

http://www.bio.tamu.edu/FACMENU/FACULTY/GoldenS.htm

Taxonomy:
Status: Nonexclusive License

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